To sort particles or cells, the cytometer first needs to identify the cells of interest, then separate out the individual cells. Once the population of interest has been identified on a data acquisition plot, a region is drawn around that population. A logical gate is created from the regions. This gate is then loaded into the cytometer’s software as the sort gate. The sort gate identifies cells of interest to be sorted out of the stream.
Different cytometers have different methods of capturing a particle of interest.(11.)
Sorting of components(11.)
